Februari 5, 2021
e-NVMS meeting with CPM contribution on glycoproteomics Covid-19 research
The e-NVMS online meeting will be held on Februari 5. This meeting is focused on Covid-19 related research. One of the speakers is Christoph Gstoettner from the CPM. Christoph will present his work on glycoproteomics Covid-19 research, titled: “Structural and functional characterization of Spike-RBD produced in different organisms“.
Januari 23, 2020
NWO LIFT project awarded: Proteoform-resolved pharmacokinetics of biopharmaceuticals
Lead applicant: Professor Manfred Wuhrer, LUMC
Consortium: LUMC, RocheDiagnostics GmbH
Modern medicines are often proteins administered into the bloodstream. These proteins will often undergo changes after this administration; for instance, they may lose part of their molecular structure, or this may become modified. The LUMC, working together with two international companies (Roche and Sciex), will devise methods to map the changes undergone by administered proteins, and then examine the influence these changes have, not just on the lifetime of the drug but also on its activity. The project will contribute towards quality control, improved drug applicability, and the development of new medicines.
November 3, 2019
Manfred Wuhrer received the Molecular & Cellular Proteomics/ASBMB Lectureship Award.
He gave the award lecture on November 3 at the Glycobiology meeting in Phoenix, Arizona.
October 25, 2019
Center for Proteomics & Metabolomics LUMC and Amsterdam UMC awarded the AR&D Grant of € 50.000,-
Together with Amsterdam UMC colleagues, Maurice van den Hoff (Medical Biology) and Sandra van Vliet (Molecular Cell Biology and Immunology), Bram Heijs was awarded the AR&D grant of € 50,000.00 on the topic: “The glycosylation status of Follistatin-like 1 regulates development and growth”. The project will officially kick-off on the 1st of November 2019 and the project team will be expanded with the addition of Mariacristina Festa. During this study the glycosylation of Follistatin-like 1 will be explored making use of high-end analytical tools available within the CPM. Moreover, based upon these results the distribution of different Follistatin-like 1 glycoforms will be studied in different organs during development and it will be investigated whether a correlation can be found between gene expression levels and patterns of glycosylation-related genes.
October 3, 2019
NWO KLEIN-1 project awarded: Cleaving like a Pro: PPEP (Pro-Pro endopeptidase)-regulated processes in Clostridiodes difficile
Dr. P.J. Hensbergen (Leiden University Medical Center)
Clostridioides difficile, a bacterium that causes intestinal infections, secretes an enzyme (PPEP-1) that regulates the spread of the bacterium. In doing so it cleaves surface proteins from the bacteria, proteins which are necessary for attachment. However, in the case of one of these surface proteins, we have yet to discover what it attaches to. PPEPs also occur in other bacteria, but their activity is slightly different. C. difficile also has a second PPEP, but its function is unknown. In this project we want to further unpack the PPEP-regulated processes in C. difficile, and to better understand the activity of different PPEPs.
July 5, 2019
Mini symposium Glycobiology at the LUMC and beyond
Glycobiology is becoming increasingly important in the majority of fundamental and translation biomedical research topics. Glycans play essential roles involved in many biological processes in cardiovascular biology, immunology, cancer and infectious diseases. To highlight and discuss outstanding glycobiology research activities by LUMC and its collaborators we like to welcome you to participate and interact with us at the minisymposium: “Glycobiology at the LUMC and beyond”.
More information and registration details can be found in the flyer.
May 21, 2019
The Center for Proteomics and Metabolomics will be opened officially at 4 pm on the 21th of May.
May 13, 2019
Method for the highly sensitive analysis of protein N-glycosylation published in Nature Communications
A new method for the highly sensitive characterization of protein N-glycosylation was developed at the CPM and resulted in a publication in Nature Communications on 13 May 2019.
The newly developed technology by Guinevere Lageveen-Kammeijer, Noortje de Haan, Pablo Mohaupt, Sander Wagt, Mike Filius, Jan Nouta, David Falck and Manfred Wuhrer allows the characterization of released N-glycans with high sensitivity, approximately 100 times higher than other state-of-the-art methods. Additionally, information is obtained on sialic acid linkage-isomers.
The researchers combined two advanced analytical techniques – capillary electrophoresis and mass spectrometry – to reach this new depth for the characterization of glycans. The hyphenated approach ensures the identification and quantification of glycans in low quantity and/or precious patient material.
To read more about the new technique and its applications please visit the website of the journal.
March 7, 2019
Guinevere Kammeijer defended her PhD thesis
On Thursday the 7th of March Guinevere Lageveen-Kammeijer defended her thesis: “Unravelling the Sugar-Coating of Prostate-Specific Antigen – Method Development and its Application to Prostate Cancer Research”.
During her PhD-project, Guinevere focused on the analysis of glycopeptides, labelled N-glycans, sialic acid differentiation as well as reaching higher sensitivities using CE-ESI-MS and how these methods could be translated to a PSA Glycomics Assay. Even though Guinevere is at the start of her career, her work got broad interest from the scientific community with more than 25 international presentations during her PhD, of which 13 on invitation. Furthermore, she received a total of 7 travel grants for several conferences and several fellowships in collaboration with the Urology department at the Amsterdam University Medical Centers (location AMC) and Astellas to support her own research line. In 2017 she joined as a member of the organization committee from the Netherlands Area Biotech (NLab) Discussion group supported by CASSS. In 2019, she became a member of the scientific committee of the glycomics session and a member of the early career committee, of MSACL EU.
Guinevere will proceed her research regarding the development of the PSA Glycosylation Assay within the Center for Proteomics and Metabolomics. Additionally, she will try to bring researchers from the field of biomarker discovery together with clinicians.
March 1, 2019
CPM moved to new location
All employees and most labs of the Center for Proteomics and Metabolomics have moved to a new location. This is the result of several years of planning and reconstruction. The new location makes it possible for the whole department to work together in a single modern office space from which the labs are quickly accessible.
Februari 27, 2019
Nature Reviews Molecular Cell Biology publication with CPM contribution
Martin Giera, head of the metabolomics group of the CPM, is co-author of a publication that appeared in Nature Reviews Molecular Cell Biology. The title of the publication is ‘Identification of bioactive metabolites using activity metabolomics‘.
Februari 14, 2019
Noortje de Haan defended her PhD thesis
The title of Noortje’s thesis is ‘Clinical Glycomics of Antibodies by Mass Spectrometry’. She defended her work at the academy building of Leiden University and graduated cum laude.
During her project, Noortje developed various mass spectrometry-based methods for the characterization of (antibody) glycosylation and applied these on samples from clinical cohorts. She was involved in studies into inflammatory bowel diseases, meningococcal sepsis and hematological stem cell transplantations. Antibody glycosylation plays an important role in the regulation of immune responses, which makes it an attractive target to study in the context of disease diagnosis and monitoring. For example, leads were found that encourage further research into glycomic changes predicting and describing inflammatory bowel diseases.
October 30, 2018
Suzanne van der Plas-Duivesteijn defended her PhD thesis
On October 30, Suzanne van der Plas – Duivesteijn defended her thesis: ‘Advancing Zebrafish Models in Proteomics’. Suzanne performed her research in a collaboration between the Center for Proteomics and Metabolomics (of the Leiden University Medical Center) and the Animal Sciences and Health department of the Institute of Biology (University of Leiden). She was supervised by Dr. Magnus Palmblad (CPM), Prof. dr. Annemarie Meijer (IBL) and Prof. dr. André Deelder (CPM).
During her PhD trajectory, Suzanne worked on the design and creation of the first zebrafish (D. rerio) spectral library. Also she designed practical applications of a software tool which was previously designed for phylogenetic clustering of mass spectrometry data. In addition the Common Carp (C. Carpio) has been studied as potential alternative model to overcome the limitations of its smaller sized close relative the zebrafish (D. rerio). In the last chapter of her thesis Suzanne described an overview of the possibilities, limitations, and practical considerations of the application of the zebrafish animal model within a proteomics design.
The created zebrafish spectral library serves the community with an enormous amount of organ specific mass spectrometry data, which is currently used in follow up research projects. The software tool CompareMS2 could be implemented in automated platforms to support high throughput data analysis. The data generated from the study to the common carp and the zebrafish library are used in the development of the software tool COMICS. This tool allows researchers to re-use available published datasets, which will contribute to the reduction of animal experiments. In addition COMICS facilitated in the understanding of tissue/organ related expression.
September 27, 2018
Frank Fleurbaaij defended his PhD thesis
Frank Fleurbaaij performed his research in a collaboration between the Department of Medical Microbiology and the Center for Proteomics and Metabolomics. Frank defended his thesis with the title ‘Novel applications of mass spectrometry-based proteomics in clinical microbiology’ in the academy building of the Leiden University.
May 25, 2018
Together with the Urology departments of the AMC and LUMC, the Center for Proteomics and Metabolomics (CPM) of the LUMC organizes a masterclass on prostate cancer, with attention to innovative technologies, with a plenary presentation given by the Spinoza Chair Awardee of 2018, Prof. K.J. Pienta (Brady Urological Institute, Johns Hopkins Hospital). Other speakers include Guinevere S.M. Kammeijer, Dr. Geertje van de Horst, Fanny Boyaval, Dr. Rudy van Eijsden. The program will start at 14:30, and will include a discussion and networking reception at the end. Attendance is free of charge but you are kindly requested to register here. For more information, see the flyer.
Februari 1, 2018
Bram Heijs new group leader for MSI
The Center for Proteomics and Metabolomics, with prof. Manfred Wuhrer at the helm, consists of five groups. From the 1st of February onwards, the Mass Spectrometry Imaging group, previously lead by dr. Liam McDonnell, will have a new leader: Bram Heijs, who will defend his PhD thesis on February 1st, 2018. The title of the thesis is “Lab-on-a-tissue: optimization of on-tissue chemistry for improved mass spectrometry imaging”. Mass spectrometry imaging is a promising, and emerging technology in the field of clinical mass spectrometry. Despite his young age, Bram Heijs has shown great knowledge and professionality which was awarded with the Imabiotech MSI award 2017 for his work on “Histology-guided high resolution mass spectrometry imaging” at the international OurConV conference for mass spectrometry imaging held in Doorn (NL) September 2017.
Januari 23, 2018
Mutanome analysis – finding neoepitopes in cancer
This minisimposium, organized by dr. van Veelen (CPM) in collaboration with the Depts. Clinical Oncology and Pathology, will give the participant the opportunity to learn about state-of-the-art strategies for finding neoepitopes for immunotherapy of cancer.
The keynote speaker is Dr. Michal Bassani (Dept. of Oncology University, Ludwig Institute for Cancer Research, Lausanne).
The symposium, held on Tuesday, January 23, 2018 from 14.00 to 17.00, will be followed by drinks, to offer an informal opportunity to make acquaintance with the people behind the science.
Please note that the location is OUTSIDE the LUMC building: Room 1A-20 FSW, building, Leiden University (Wassenaarseweg 52)
Januari 16, 2018
The hitchhiker’s guide to data funnels
Within the lecture series “LUMC TOP RESEARCH SEMINARS”, introduced to stimulate competition and to foster collaboration and inspiration/formation of new research ideas, the event of January 16, 2018 will be dedicated to TFA data analytics. Among the speakers, our very own Arzu Tugce Guler, with a presentation titled “the hitchhiker’s guide to data funnels”. The seminar starts at 17:00, in lecture room 3, with an introduction by prof. Jelle Goeman (dept. medical statistics and bioinformatics)
The work performed within the IBD-BIOM consortium, of which the CPM was one of the beneficiaries, has resulted in this publication in Gastroenterology (ranked as #1 in gastroenterology with IF=18.1). The large collaborative paper shows that IgG glycosylation is both only biomarker and functional effector in IBD (Inflammatory Bowel Disease). The article, titled “Glycosylation of Immunoglobulin G Associates With Clinical Features of Inflammatory Bowel Diseases”, is based on the study of 3,441 samples from patients with IBD and matching controls. Sample collection was coordinated by dr. Vito Annese (AOU Careggi University Hospital of Florence) and dr. Dermot McGovern (Cedars Sinai Hospital in Los Angeles). Glycan analysis was performed at Genos in Zagreb and at the CPM (LUMC).
Noortje de Haan (CPM), Mirna Šimurina (University of Zagreb) and Frano Vučković (Genos) share the first authorship of this important contribution.
IMabiotech MSI award to Bram Heijs
During the OurConV Conference for Mass Spectrometry Imaging, held in Doorn (NL) in the last week of September 2017, Bram Heijs received the ImaBiotech MSI award.
This important prize, developed by ImaBiotech four years ago, is an incentive to promote innovation in the field of Mass Spectrometry Imaging.
Bram is working in the Mass Spectrometry Imaging group of the CPM on methods for on-tissue digestion MALDI-MSI and on the application of optimized methods to characterize and differentiate various types of clinical samples. He will defend his thesis, titled “Lab-on-a-tissue: optimization of on-tissue chemistry for improved mass spectrometry imaging”, in early 2018. Since October 2017 he is the new MSI group leader within the Center for Proteomics and Metabolomics.
September 29, 2017
Clinical Mass Spectrometry Imaging Symposium
This mini‐symposium on Clinical Mass Spectrometry Imaging will give you the opportunity to learn more about the application of this cutting edge technology in clinical research.
The program will include keynote lectures by outstanding experts in the field, as well as by young promising researchers.
The symposium will be followed by lunch in “Het Paleijhs” (in front of the lecture room), to offer an informal opportunity to make acquaintance
with the people behind the science.
Click here for the flyer, including the full program.
June 6, 2017
Former CPM employee is awarded the International Glycoconjugate Organization (IGO) Young Glycoscientist Award 2017
Dr. Yoann Rombouts has been awarded the International Glycoconjugate Organization (IGO) Young Glycoscientist Award 2017, a prestigious price to reward young estabilished glycoscientists who have made pioneering contributions in the field of glycoscience and show great promise for further achievements. The IGO recognized the high value of Dr. Rombouts’ PhD on mycobacterial glycoconjugates (Lille, France) and his postdoctoral work on glycosylation of rheumatoid arthritis-specific autoantibodies (LUMC). During his appointment at the LUMC, dr. Rombouts’ line of research encompassed work carried out both at department of Rheumatology and at the Center for Proteomics and Metabolomics.
Dr. Rombouts will receive the Prize at the 24th International Symposium on Glycoconjugates in Jeju, Korea.
May 11, 2017
Clinical Proteomics and Metabolomics
Thursday, May 11, 2017
17:00, Lecture Room 5
The CPM (Center for Proteomics and Metabolomics) is one of the Technology Focus Areas within the LUMC. On Thursday, May 11, from 17:00 to 19:30 we will have our second CPM research evening, presenting the research focus of the CPM, with particular attention to its collaborations within the LUMC. The title of the event
(held in Lecture Room 5, building 1, LUMC) is: Clinical Proteomics and Metabolomics.
We will highlight selected research topics of the five groups which are active in the CPM (Metabolomics, Proteomics, Glycomics, MS Imaging and Bioinformatics). Particular attention will be dedicated to collaborations within the LUMC, as Prof. Toes (Rheumatology department) will be one of the speakers. The aim is to introduce the CPM, its research, and its facilities to other researchers within and outside the LUMC, and to identify new research opportunities. The symposium will be followed by drinks in Het Paleijhs (in front of the lecture room), to offer an informal opportunity to make acquaintance with the people behind the science.
Click here for the flyer, including the full program.
October 25, 2016
Horizon 2020 funding for advancing personalized medicine
Lots of research is being done on discovering biomarkers for various diseases to improve diagnostics and therapy, but very few of those biomarkers are actually being used in clinical practice. Specific sugar signatures of diseases are particularly promising for guiding clinicians in their decisions for a personalized healthcare approach. A European consortium, coordinated by the LUMC, is now receiving EU-funding for a successful translation of promising sugar biomarkers into clinical practice by collaborating intensively with healthcare industry.
The European Horizon 2020 program is funding the Marie-Curie European Industrial Doctorates project GlySign. The major goal of the academic-industrial consortium employing six PhD students is to establish clinical assays that are simple, fast and reliable enough to be used in routine clinical analysis. To this end, already known sugar (or glycan) signatures of four different diseases – diabetes, prostate cancer, neonatal alloimmune thrombocytopenia, and rheumatoid arthritis – will be used.
Prof. Manfred Wuhrer, head of the Center for Proteomics and Metabolomics at the LUMC, is the initiator of this project. He has many years experience in the development of analytical methods for the analysis of complex sugars that are attached to proteins in patients’ blood and other samples. These sugar molecules fine-tune the proteins’ functions and can therefore reflect specific changes caused by diseases and other body conditions, such as pregnancy and even a person’s biological age or sex.
The GlySign project will not only train six early-stage researchers in clinical glycan analysis technologies: Likewise, the knowledge and personnel exchange between the clinical, academic and industrial partners will promote the clinical use of glycan markers in disease diagnosis and prognosis. Different training formats, such as regular scientific meetings, internships at the partner institutions as well as business-oriented and scientific workshops, will support these goals. The required steps for the implementation of the developed analytical technologies will be achieved in close collaboration with healthcare industry that are expert in the production of standardized, high-quality assays for their use in routine clinical laboratories. By this, GlySign will contribute to the development of improved, personalized diagnostic and therapeutic approaches for different diseases.
September 15, 2016
Article on antibody glycosylation in rhesus disease treatment wins 2016 RISE award
The article “Prophylactic anti-D preparations display variable decreases in Fc-fucosylation of anti-D” (DOI: 10.1111/trf.12880) has been selected as the best paper published in 2015 in TRANSFUSION’s , and is thus, winner of the RISE Award. This award (Research Innovation in Scientific Excellence) recognizes impeccable study design, innovation, significance, and effective communication in any area of knowledge covered by the journal.
The paper is the result from a collaboration between Sanquin (Dr. G. Vidarsson, Prof. Dr. Ellen van der Schoot) and LUMC/CPM (Prof. Dr. M. Wuhrer). Sample analysis by mass spectrometry was performed by Agnes Hipgrave Ederveen (CPM). The results will contribute to a better understanding of rhesus D hemolytic disease of the newborn, and future research will aid immunoprophylaxis optimization.
The 2016 RISE Award will be presented at the Annual AABB Meeting Opening Ceremony on Saturday, October 22, 2016 in Orlando.
August 30, 2016
in the clinical environment symposium
Thursday, September 22, 2016
17:00, Lecture Room 5, LUMC
The CPM (Center for Proteomics and Metabolomics) is one of the Technology Focus Areas within the LUMC. On September 22, from 17:00 to 19:00 you will have the opportunity to learn more about the research focus of the CPM, with particular attention to its collaborations within the LUMC. The title of the symposium (held in Lecture Room 5, building 1, LUMC) is: Mass spectrometry in the clinical environment.
It will highlight the research topics of the five different groups which are active in the CPM (Metabolomics, Proteomics, Glycomics, MS imaging and Bioinformatics). The aim is to introduce the CPM, its research, and its facilities to other researchers within and outside the LUMC, and to identify new research opportunities. The symposium will be followed by drinks in Het Paleijhs (in front of the lecture room), to offer an informal opportunity to make acquaintance with the people behind the science.
Click here for the flyer, including the full program.
May 9, 2016
Glycosylation in colorectal cancer: LUMC coordinator of EU-funded consortium
Colorectal cancer (CRC) is the third most common cancer in the world, and the Netherlands are among the top 5 countries with the highest CRC incidence. To advance improvements in early detection and therapy of CRC, the international Marie-Curie European Training Network GlyCoCan (https://glycocan.eu/) will train 13 PhD students in an interdisciplinary environment with substantial industrial involvement. Within the H2020 scheme, the project was awarded € 3.2 million, whereof the LUMC, as coordinating institution, receives almost € 800,000.
CRC screening and glycan research
The treatment of CRC is most successful when detected at an early stage. Therefore, population-based screening of CRC is thought to reduce mortality and healthcare costs. Currently available screening methods, such as the fetal occult blood test, lack sensitivity, specificity or user-friendliness. Glycans (complex sugars) are known to reflect pathological changes, especially in cancer including CRC. However, glycoscience is a young discipline and the structural analysis of glycans is still a major challenge hampering its translation into medical practice. Professor Manfred Wuhrer, head of the Center of Proteomics and Metabolomics at LUMC, is pioneering the field of clinical glycan analysis. He has assembled a consortium of 15 European partners that are all experts in either glycoscience or CRC research.
Multilevel training and research approach
Since CRC incidence is estimated to double within the next 20 years, there is an urgent need for experts to fight this global threat. GlyCoCan as a European Training Network will address this on multiple levels, by focusing on CRC glycobiology and glyco-immunology, as well as at the clinical application of promising diagnostic glycan targets. Uniquely, clinicians, industrial partners and scientists from 8 different European countries will join forces to promote glyco-oncology in the healthcare sector both in Europe and worldwide. By enhancing our understanding of the structure-function relationship of glycans in CRC, GlyCoCan will pave the way for the development of improved diagnostic and prognostic biomarkers and novel therapeutic targets.
April 20-22, 2016
Glycobiology in Health and Disease seminar
April 20-22 the LUMC will host the Glycoscience Japan – The Netherlands Joint Seminar 2016.
The theme of this 5-yearly meeting is “Glycobiology in Health and Disease” and more than 35 Japanese and Dutch invited speakers will present their most recent work in this exciting area of research.
Themes include Immunology, Cancer, Development, Genetic Disorders, Chemical Glycobiology and Host-Pathogen Interactions. Many interested in glycobiology specifically have applied for the entire 3-day meeting.
However, in particular for biomedical and medical researchers at LUMC it may be interesting to attend selected presentations and learn more about glycobiology in your research field.
Please feel free to join for individual sessions/presentations of interest to you, without registration.
The full program can be found here.
november 13, 2015
NPP Fall meeting
The 2015 NPP Fall meeting is hosted by the CPM at the LUMC. The program of the meeting can be found the NPP website.