The Center for Proteomics and Metabolomics (CPM) forms the main expertise center and technology focus area for (clinical) proteomics and metabolomics in the LUMC. The key technologies are liquid separations (liquid chromatography and capillary electrophoresis), gas chromatography, mass spectrometry (MS) and nuclear magnetic resonance (NMR). Our main field of expertise is the application of these technologies in clinical research.
The Center houses over 30 state-of-the-art mass spectrometers ranging from triple-quadrupole and ion trap instruments to time-of-flight and Fourier transform mass spectrometers. Most of the instruments are coupled on-line (electrospray ionization, ESI) or off-line (matrix assisted laser desorption/ionization, MALDI) to liquid separations. The MALDI instruments are used for high-throughput screening and imaging mass spectrometry, whereas the ESI mass spectrometers are used in a wide range of proteomics and metabolomics research projects for identification and quantitation of proteins, peptides and small molecules.