PhD Sarantos Kostidis
Sarantos Kostidis is a researcher specialising in metabolomics methodologies to study cellular and organ metabolism. His work consist of complete workflows, from project design to the setup of experimental protocols for optimum sample analysis, quantification of metabolites, and the interpretation and visualization of generated data.
He is involved in several partnerships with both academia and industry in studies such as, the metabolic rewiring of tumor cells under hypoxia and reoxygenation, the metabolic adaptation of T cells under different stimuli, the integrated phenotypic profiling of epithelial cells upon enzymatic inhibition, and the metabolic and lipidomic characterization of differentiated human iPSCs (cardiomyocytes, kidney endothelial cells and brain cell types such as microglia, neurons and astrocytes).
Sarantos current research focus is in setting up and applying methodologies for Stable Isotope Tracing and Flux analysis of cells and organs.
Sarantos has studied chemistry in the University of Crete and obtained his PhD in chemistry and biomolecular NMR from the University of Ioannina (Greece). In 2007 he joined the Pharmaceutical Chemistry lab at the Department of Pharmacy of the University of Athens, where he started working in the field of Metabolomics. In 2012 he moved to Leiden to join the Biomolecular NMR group of LUMC, currently Center for Proteomics and Metabolomics.
He is author and co-author of more than 35 research papers, serves as a reviewer for several journals like Analytical Chemistry, Analytical Chimica Acta, and J. Proteome Res., lecturer in workshops and participant in funding proposals. He is also a member of external PhD evaluation committee and involved in daily supervision of PhD, MSc and BSc students.
Quantitative NMR analysis of intra- and extracellular metabolism of mammalian cells: A tutorial.
Kostidis S, Addie RD, Morreau H, Mayboroda OA, Giera M. Anal Chim Acta. 2017; 980: 1-24. (doi: 10.1016/j.aca.2017.05.011)
Anal Chim Acta. 2017; 980: 1-24. (doi: 10.1016/j.aca.2017.05.011)
Results of an explorative clinical evaluation suggest immediate and persistent post-reperfusion metabolic paralysis drives kidney ischemia reperfusion injury.
Lindeman JH, Wijermars LG, Kostidis S, Mayboroda OA, Harms AC, Hankemeier T, Bierau J, Sai Sankar Gupta KB, Giera M, Reinders ME, Zuiderwijk MC, Le Dévédec SE, Schaapherder AF, Bakker JA.
Kidney Int. 2020; 98(6):1476-1488. (doi: 10.1016/j.kint.2020.07.026)
Shear Stress Regulation of Endothelial Glycocalyx Structure Is Determined by Glucobiosynthesis.
Wang G, Kostidis S, Tiemeier GL, Sol WMPJ, de Vries MR, Giera M, Carmeliet P, van den Berg BM, Rabelink TJ.
Arterioscler Thromb Vasc Biol. 2020; 40(2): 350-364. (doi:10.1161/ATVBAHA.119.313399)