STANDARD ANALYSES/TECHNIQUES WE APPLY INCLUDE:
- Proteomics and (HLA)-Peptidomics
- Relative protein quantification (SILAC, Dimethyl labelling, TMT labelling, label free)
- Identification of post-translational modifications (e.g. glycosylation, phosphorylation, ubiquitination, deamidation, citrullination, carbamylation)
- Quantitation by MRM/PRM
HIGHLIGHTS OF OUR RESEARCH
- The elucidation of various T cell epitopes relevant to celiac disease, cancer, diabetes and transplantation
- Fundamental cell biology and antigen processing studies with Dept. Cell and Chemical Biology
- The role of miRNAs in the development of lymphocytes and their relevance to the development of acute myeloid leukemia (AML), with Dept. Hematology of the Erasmus MC in Rotterdam
- The role of post-translational modifications in rheumatoid arthritis, in particular of carbamylation
- The elucidation of a novel proline specific metalloprotease (Pro-Pro endopeptidase, PPEP-1) in Clostridioides difficile (with Dept. Medical Microbiology)
- Clostridioides difficile phosphoproteomics (with Dept. Medical Microbiology)
- Glycoproteomics of cancer cells
- The elucidation of two new enzymes, nardilysin and TOP, shown to be involved in antigen processing, i.e. in the creation of epitopes/HLA-ligands. This finding adds an extra level of complexity to antigen processing
FINANCIAL SUPPORT
Dutch Research Council (NWO), European Union, Dutch Cancer Society (KWF) and the LUMC.
COLLABORATIONS
Currently we have collaborations with many groups within the LUMC (e.g. Immunology, Hematology, Rheumatology, Clinical Oncology, Cell and Chemical Biology, Anatomy, Human Genetics, Pathology, Infectious Diseases, Medical Microbiology) but also with research groups from other universities and research institutes, e.g. NKI, RIVM, in the Netherlands and abroad. In addition, we collaborate with industrial partners.
