Our primary focus is on method development to improve the depth of identification of proteomes and peptidomes to enable a better understanding of mechanisms in health and disease. Also, we use these methodologies to elucidate protein function e.g. of proteases. To this end, a wide variety of chromatographic (C18, SCX, AEX, HILIC, TiO2, affinity chromatography) and electrophoretic (SDS-PAGE, CE, IEF) techniques is available. The available mass spectrometric equipment comprises a Q-Exactive, LTQ-FT Ultra, several amaZon ion traps -including ETD-, and an UltrafleXtreme.