Mass spectrometry-based proteomics has become an invaluable tool in modern biomedical research. The proteomics group performs state-of-the art mass spectrometric analyses of peptides/proteins, with an historical focus on immunology, i.e. HLA-peptidomics and antigen processing, cell biology and microbiology together with our collaborators.
Our primary focus is on applying and improving the depth characterization of proteomes and peptidomes, and their post-translational modifications, to enable a better understanding of mechanisms in health and disease. To this end, we combine a variety of peptide and protein sample preparation techniques with nanoLC-MS/MS analysis. The available mass spectrometric equipment comprises several Orbitraps™ (LUMOS Tribrid™, Exploris480™ and ASTRAL™).
STANDARD ANALYSES/TECHNIQUES WE APPLY INCLUDE:
- Proteomics and (HLA)-Peptidomics
- Relative protein quantification (SILAC, Dimethyl labelling, TMT labelling, label free)
- Identification of post-translational modifications (e.g. glycosylation, phosphorylation, ubiquitination, deamidation, citrullination, carbamylation)
- Quantitation by MRM/PRM
