Assistant Professor / Group leader Proteoform Profiling
Dr. Elena Dominguez Vega
DETAILS
I am assistant professor and group leader of the Proteoform Profiling group. My scientific interests focus on the development and application of advanced mass spectrometric methods for the structural and functional characterization of (glyco)proteins of clinical and pharmaceutical interest. In particular, I aim to study proteins at the proteoform level to better understand their functional diversity. To this end, we utilize a variety of separation techniques, typically operated in the low-flow regime, hyphenated with MS to resolve molecular characteristics of proteins at the intact level. Recently, my group has developed a proteoform-specific binding assay based on affinity capillary electrophoresis-MS, enabling the study of proteoform-specific interactions in complex mixtures. This information can help understanding molecular mechanisms of proteoforms in health and disease and contribute to the development of better therapies.
CURRICULUM VITAE
2021-present: Assistant Professor and Group Leader of the Proteoform Profiling group at the CPM
2021- present: Board member of the CE Discussion Group, part of the KNCV
2017-2021: Senior Researcher, Center for Proteomics and Metabolomics, Leiden University Medical Center
2019-2022: Founder and board member of the Spaanse Wetenschappers in Nederland (CENL-SWNL)
2013-2017: Postdoctoral researcher, Division of BioAnalytical Chemistry, VU University Amsterdam, Amsterdam
2012-2013: Postdoctoral researcher, Biomolecular Analysis group, Utrecht University, Utrecht
2007-2011: PhD candidate, Department of Analytical Chemistry, University of Alcalá, Spain
Publications
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Fc Profiling of Polyclonal IgG, IgA and IgM by Light Chain Capturing Coupled with NanoRP-LC-MS
Yue Li, Sabrina Reusch, Bianca DM van Tol, Fiammetta Di Marco, Anna M Wasynczuk, Steinar Gijze, David Falck, Manfred Wuhrer, Constantin Blöchl, Christoph Gstöttner, Elena Domínguez-Vega.
JACS Au, 2026, 6, 644−652. https://doi.org/10.1021/jacsau.5c00839
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Fc Proteoforms of ACPA IgG Discriminate Autoimmune Responses in Plasma and Synovial Fluid of Rheumatoid Arthritis Patients and Associate with Disease Activity.
Constantin Blöchl, Eva Maria Stork, Hans Ulrich Scherer, Rene E. M. Toes, Manfred Wuhrer, Elena Domínguez-Vega.
Advanced Science, 2025, 12, 2408769. https://doi.org/10.1002/advs.202408769
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Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner
Christoph Gstöttner, Alexander Knaupp, Gestur Vidarsson, Dietmar Reusch, Tilman Schlothauer, Manfred Wuhrer and Elena Domínguez-Vega.
Frontiers in Immunology 2022, 13, 980291. https://doi.org/10.3389/fimmu.2022.980291
Groups:
Proteoform Profiling
