Assistant Professor / Group leader Tissue Glycomics
Dr. Noortje de Haan
DETAILS
I am assistant professor at the Center for Proteomics and Metabolomics (CPM) at the LUMC, leading the Tissue Glycomics group. I’m passionate about the development of analytical, mass spectrometry-based techniques to study glycans (complex carbohydrate structures covering the outside of all human cells) and proteins in human tissues and revealing the role of glycans in human (patho)physiology. Glycans, compared to other biomolecules, stand out for me due to their chemical complexity and strong implications in, amongst others, immunology, regenerative medicine, virology, and – importantly- oncology. This interdisciplinarity is what excites me and drives me to combine glycobiology with cutting-edge mass spectrometry (MS) to identify and exploit specific roles of glycoproteins in human (patho)physiology. The core technologies in the lab for these purposes are nano liquid chromatography coupled to mass spectrometry (nanoLC-MS) and mass spectrometry imaging (MSI).
During my PhD research (cum laude, 2019) in the lab of Prof. Manfred Wuhrer, I worked on mass spectrometry method development and clinical glycomics with a core focus on antibody glycosylation in inflammatory diseases. I expanded my expertise in tissue glycomics during my three year postdoc in the glycobiology lab of Prof. Hans Wandall at the University of Copenhagen (Denmark), where I got trained in the biochemical and genetic aspects of glycosylation and advanced mass spectrometry for glycomics and glycoproteomics.
CURRICULUM VITAE
2023-present: Assistant professor and group leader of the Tissue Glycomics group at the Center for Proteomics and Metabolomics, Leiden University Medical Center, The Netherlands.
2023-present: Visiting researcher at the Copenhagen Center for Glycomics, University of Copenhagen, Denmark.
2020-2023: Postdoctoral researcher at the Copenhagen Center for Glycomics, University of Copenhagen, Denmark.
2020-present: Organizing member of the European Glycoscience Community, co-organizing and hosting the analytical glycoscience sessions of the bimonthly webinar series.
2018-2020: Postdoctoral researcher at the Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.
2014-2018 PhD-student at the Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands. Thesis: Clinical Glycomics of Antibodies by Mass Spectrometry (cum laude)
2012-2014 Master program Biomarkers and Clinical Chemical Analysis at the VU university, Amsterdam, The Netherlands
2012-2014 MSc+ Program Analytical Chemistry, at TI-COAST, Amsterdam, The Netherlands
2009-2012 Bachelor program Pharmaceutical Sciences at the VU university, Amsterdam, The Netherlands
Publications
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Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line.
M Nielsen*, N de Haan*, W Kightlinger, Z Ye, S Dabelsteen, M Li, M Jewett, I Bagdonaite, S Vakhrushev, HH Wandall * Equal contributing authors
Nature Communications, 2022, Nature communications 13 (1), 6257 https://doi.org/10.1038/s41467-022-33806-8
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In-Depth Profiling of O-Glycan Isomers in Human Cells Using C18 Nanoliquid Chromatography–Mass Spectrometry and Glycogenomics
N de Haan, Y Narimatsu, M Koed Møller Aasted, ISB Larsen, IN Marinova, S Dabelsteen, SY Vakhrushev, HH Wandall
Analytical Chemistry, 2022, 94 (10) 4343-4351. https://doi.org/10.1021/acs.analchem.1c05068
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Sensitive and Specific Global Cell Surface N-Glycoproteomics Shows Profound Differences Between Glycosylation Sites and Subcellular Components.
N de Haan, M Song, OC Grant, Z Ye, F Khoder Agha, M Koed Møller Aasted, RJ Woods, SY Vakhrushev, HH Wandall
Analytical Chemistry, 2023, 95 (47), 17328-17336 https://doi.org/10.1021/acs.analchem.3c03626
Groups:
Tissue Glycomics
